Sucrose preference test was performed as described previously63. Briefly, 10-week-old male mice on chow diet (n=6 each group; body weight, 24.68±0.77 g for WT and 23.73±0.89 g for FoxO1 KODAT; P>0.4) were used. Mice were housed in cages equipped with two drinking bottles, one containing filtered tap water and another one containing sucrose solution. The mice were first trained and acclimatized to the new environment for 3 days and then the sucrose preference test was performed using different concentrations of the sucrose solutions, 1, 2 and 5% (each concentration was tested for 3 days). The mice had free access to both bottles during the habituation and the experimental period. Fluid consumption was measured twice a day at 8:00 and 17:00 by weighing the bottles. To avoid side bias, the positions of the bottles were switched daily. On the last day of experiment period, mice were fasted overnight (with water ad libitum) and sucrose preference test was subsequently monitored with 2% sucrose. Sucrose preference was calculated as the percentage of the amount of sucrose solution consumed over total fluid consumption.
