Chunk #9 — Materials and Methods — Marker selection, genotyping and error checking

Source

Interaction between two independent CNR1 variants increases risk for cocaine dependence in European Americans: a replication study in family-based sample and population-based sample.

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Genomic DNA was obtained from immortalized cell lines, blood or saliva. All SNPs were genotyped by a fluorogenic 5’ nuclease assay [Taqman technique; (Shi et al 1999)]. Mendelian inconsistencies in family genotype data were identified by implementing Pedcheck (O'Connell and Weeks 1998). One inconsistency was found and the genotype data for this entire pedigree were excluded from this study. Hardy-Weinberg Equilibrium (HWE) was tested using the program PowerMarker (Liu and Muse 2005).