The deactivation of GluA1 homomers in the presence of both γ-8 and CNIH in HEK cells is at least as slow as that observed with CNIH alone (Figure 6Ai). Furthermore, the IKA/IGlu ratio of GluA1 homomers is the same when expressed in HEK cells with only γ-8 and when CNIH-2 and γ-8 are co-expressed (Figure 6Aii). Therefore, these receptors must be associated with at least 1 and possibly 4 CNIH molecules, in addition to the 4 γ-8 (Figure 8A). We cannot be more precise on the CNIH stoichiometry but γ-8 and CNIH-2 are capable of co-occupying GluA1 subunits.
