In HEK cells, based on the same fast kinetics of GluA2 homomers in the presence of both γ-8 and CNIH or with γ-8 alone (Figure 6B), we propose that γ-8 prevents GluA2 subunits from associating with CNIH, with GluA2 homomers containing 4 γ-8 and 0 CNIH (Figure 8B). This model is supported by the ability of GluA2A3 receptors to coimmunoprecipitate γ-8 but not CNIH-2 from hippocampal lysates (Figures 3I and S4D).
