The HPA contains images of histological sections from normal (Tissue Atlas) and cancer tissues (Human Pathology Atlas) obtained by IHC. Since specimens are derived from surgical material, normal was defined as non-neoplastic and morphologically normal. Tissue microarrays were used to show ZDHHC5 antibody (Atlas Antibodies Cat#HPA014670, RRID:AB_2257442) staining in samples from 3 individuals defined as normal lung tissue, and samples from 6 cancer patients corresponding to 12 LUAD samples. Each sample was represented by 1-mm tissue cores. Basic annotation parameters included an evaluation of (i) staining intensity (negative, weak, moderate, or strong), (ii) fraction of stained cells (<25, 25–75, or > 75%), and iii) subcellular localization (nuclear and/or cytoplasmic/membranous). The histological subtype was classified by a clinical pathologist in our group, and semiquantitative estimation of the different histological patterns present in 5% increments according to the 2015 WHO Classification of Lung Tumors (Travis et al., 2015). LUAD is classified according to the most representative pattern in the cross-sectional area of the tissue section (the so-called main pattern), and pathological types are introduced according to the main pattern (Kuhn et al., 2018).
