Dorsolateral Prefrontal Cortex: Total RNA from 469 HBCC samples was isolated from approximately 100 mg pulverized tissue from each sample by Trizol/chloroform extraction and purification with the Qiagen RNeasy kit (Cat #74106) according to manufacturer’s protocol. Samples were processed in randomized batches of 12. The order of extraction was assigned randomly with respect to diagnosis and all other sample characteristics. The mean total RNA yield was 24.2 ug. The RNA Integrity Number (RIN) was determined by fractionating RNA samples on the 4200 Agilent TapeStation System. Samples with RIN > =5.5 were included in the sequencing set, resulting in 69 samples with RIN < 5.5 to be excluded from the study. An additional 11 samples were removed post sequencing due to sample genotype variant inconsistencies, and 4 duplicate samples were removed (the sample with the highest RIN was kept) resulting in a final dataset of 385 samples.
