The following primer pairs were used for PCR amplification: gpb-1 (5'-ATGAGCGAACTTGACCAAC-3' and 5'-TTAATTCCAGATCTTGAGG-3'), par-1 (5'-CAAAGCACGTGATAACCGG-3' and 5'-TTGGTGGCTCAATAAATGGC-3'), par-3 (5'-TTTGGCTTCACTGTGACCG-3' and 5'-TGATGTGCTGTGGATCAGC-3'), par-2 (5'-GCCGTCGCCCACTGTCG-3' and 5'-CCGGCTCCAGAGTGTCC-3'), cyk-1 (5'-GAAGAACAGCTGACCAGCG-3' and 5'-GACGATTCAATGCAATGATGG-3'), skn-1 (5'-CTGCCGAAGAGAATGCTCG-3' and 5'-GTTTGGTACAACTTCTGTTGG-3'), dnc-1 (5'-TCTCCACTTTCTACTACAGC-3' and 5'-TGTTCTTGGAAGCCAGCG-3'), bir-1 (5'-ATGGCACCCGGGACCAA-3' and 5'-TTATTTGCCGCGGCGGC-3'), pal-1 (5'-GGGGTACCCCAATGTCGGTCGATGTCAAGTCG-3' and 5'-CATGCCATGGCATGGTACTTATAGCCGAATCTTCTG-3'), dif-1 (5'-ACGCATTGAAATGTCGGACG-3' and 5'-TTGCAGGGAAAGCACGGAG-3'), and plk-1 (5'-GACAAGGATCGTGGGACC-3' and 5'-AGCACAGCAACTTGGTGG-3'). Primer pairs for mex-3, dhc-1, par-6, unc-37, hlh-2, mei-1 and rba-2, the 88 genes on cosmids K04G2 to R05D11, and the 1,200 genes used for large-scale comparison of feeding and injection were obtained as part of the Research Genetics C. elegans GenePairs collection. Fragments corresponding to unc-22 and GFP were generated from L4440-based vectors containing those inserts (pLT 61.1 and pPD128.110, respectively) [4].
