Chunk #30 — Materials and methods — Bacterial induction method tests

Source

Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans.

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Single colonies of HT115 bacteria containing cloned L4440 plasmids were picked and grown in culture in LB with 50 μg/ml ampicillin (Amp), except where indicated. The following methods were used to induce expression of dsRNA: