Analysis of synapsembles requires specific manipulations of the connectivity between different neurons types (von Engelhardt et al., 2010; Wulff et al., 2009). Synapsembles can be also targeted by fast optical means (Wang et al., 2007; Szobota et al., 2007) so that transitions from words to words can be affected. While it is impossible to be prophetic in this fast developing area of research (O’Connor et al., 2009; Miesenböck 2009), it is likely that the combination of large-scale multiple single neuron recordings and application of a whole family of perturbation methods will open new possibilities for understanding the content and meaning of assemblies and their sequential organization.
