Since microRNAs are known to affect multiple targets (Ambros 2004, Bartel 2004), we wondered whether alcohol regulated other miR-9 targets. A search by miRNA target prediction software - TargetScan (Release 4.1, Grimson et al., 2007) retrieved 826 predicted miR-9 targets (as of January 18, 2008). From this catalog we selected a list of targets (Table 1) with known roles in alcohol’s actions, and documented expression in the CNS (12 total, see Methodology).
