Iba1 immunostaining (microglia/macrophage marker) and NE immunostaining (neutrophil marker) were detected at 6 hours in the LPS-injected brains (Fig. 5). Iba1 positive cells showed the typical shape of microglia and almost of them exhibited IL-6 immunostaining (Fig. 5A). On the contrary, ~80% of IL-6 positive cells were Iba1 positive. NE immunoreactivity coincided with iNOS immunoreactivity (Fig. 5B). Iba1-positive cells and NE-positive cells were seen more pronouncedly at 3 days after LPS injection (Fig. 6A). Wy-14643 and fenofibrate dramatically decreased the numbers of these positive cells (Figs. 6A–C). Similar effects by Wy-14643 and fenofibrate were seen on the number of Isolectin B4 positive cells (Fig. 6D).
