The reagents were obtained from indicated suppliers: wheat germ lectin (WGL)-Sepharose 6MB from Amersham Pharmacia (Uppsala, Sweden); [35S]L-methionine/[35S]L-cysteine (1175.0 Ci/mmol) from PerkinElmer Life Sciences; Dulbecco's modified Eagle's medium without L-methionine and L-cysteine from Invitrogen (Carlsbad, CA); Triton X-100 from Sigma-Aldrich (St. Louis, MO); Geneticin from Mediatech (Herndon, VA); Hind III and other restriction enzymes from Promega (Madison, WI); Immobilon-P PVDF transfer membrane from Millipore Corporation (Billerica, MA); Mini Complete™ Protease Inhibitor Cocktail was from Roche (Nutley, NJ); HA.11, a product of Covance (Cumberland, VA); PNGase F and relevant buffers from New England Biolabs (Ipswich, MA); 0.2-μm spin cartridges from Rainin Co., (Emeryville, CA); SuperSignal West Pico Chemiluminescent Substrate Solutions from Pierce Co. (Rockford, IL). Reagent-grade chemicals were purchased from Sigma-Aldrich (St. Louis, MO) or Fisher (Pittsburgh, PA).
