Chunk #12 — Results — hiPSC-Astrocytes and Primary Human Fetal Astrocytes Share Similar Transcriptional Profiles

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An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells.

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identified a group of 19 genes related to reactivity, cytokine, interferon, T cell receptor (TCR), and antigen-processing signaling that were enriched in astrocytes (G2) (Figures 3A and S2C; Table 2).Table 2Annotation of the Top 100 Most Variable GenesMSigDBGroupOverlap SizeGroup SizeMSigDB SizeFEp ValueFDROverlap GenesAntigen processing and presentationG271957151.31.71 × 10−148.49 × 10−11CD74; HLA-DOA; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB5Cell adhesion molecules (CAMS)G261911564.33.17 × 10−103.61 × 10−7HLA-DOA; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB5Reactome MHC class II antigen presentationG25198671.66.64 × 10−96.94 × 10−6CD74; HLA-DOA; HLA-DQA1; HLA-DRB1; HLA-DRB5Reactome translocation of ZAP70 to immunological synapseG231975281.58 × 10−81.53 × 10−5HLA-DQA1; HLA-DRB1; HLA-DRB5Reactome phosphorylation of CD3 and TCR ζ chainsG231910369.65.42 × 10−84.85 × 10−5HLA-DQA1; HLA-DRB1; HLA-DRB5Reactome PD1 signalingG2319113367.45 × 10−86.22 × 10−5HLA-DQA1; HLA-DRB1; HLA-DRB5Reactome generation of second messenger moleculesG231918205.33.67 × 10−70.000287HLA-DQA1; HLA-DRB1; HLA-DRB5Reactome downstream TCR signalingG231927136.91.31 × 10−60.000965HLA-DQA1; HLA-DRB1; HLA-DRB5Neuron differentiationG14287345.81.68 × 10−60.001205CNTN4; LMX1B; OTX2; SLIT1Generation of neuronsG14287942.32.31 × 10−60.001574CNTN4; LMX1B; OTX2; SLIT1Axon guidanceG1328221142.32 × 10−60.001574CNTN4; OTX2; SLIT1NeurogenesisG14288937.63.73 × 10−60.002458CNTN4; LMX1B; OTX2; SLIT1Nervous system developmentG162836613.73.95 × 10−60.002538CNTN4; LMX1B; LY6H; OTX2; SHOX2; SLIT1Reactome TCR signalingG23194190.14.74 × 10−60.002876HLA-DQA1; HLA-DRB1; HLA-DRB5Nervous system developmentS151736618.84.82 × 10−60.002876CNTN4; LMX1B; LY6H; SHOX2; SLIT1Biocarta TCRA pathwayG22195492.86.23 × 10−60.003549HLA-DRA; HLA-DRB1Reactome co-stimulation by the CD28 familyG23195271.19.77 × 10−60.005322HLA-DQA1; HLA-DRB1; HLA-DRB5Reactome interferon-γ signalingG23195764.81.29 × 10−50.006553HLA-DQA1; HLA-DRB1; HLA-DRB5Biocarta IL-5 pathwayG221973521.31 × 10−50.006553HLA-DRA;