Human ESC-derived MGE cells in transplantation medium (HBSS with 10ng/ml GDNF, 10ng/ml BDNF and 20 µM Boc-Asp (OMe) fluoromethyl ketone (BAF)) were injected into hippocampus of mice using a Leica Angle Two digital stereotaxic instrument fitted with a Cunningham Mouse Adaptor. Nod-Scid mice were anesthetized using an induction chamber supplied with 4–5% isoflurane mixed with 0.8-1 L/min oxygen using a calibrated vaporizer. Animals were then administered continuous isoflurane (1–2%) mixed with oxygen (0.8–1.0 L/min) for the duration of anesthesia. Cells were transplanted into the following coordinates: AP 1.75 mm, L ±2.3 mm, V −1.7 mm for the rostral CA3 site; AP 3.25 mm, L ±3.0 mm, V −3.65 mm, −2.9 mm and −2.0 mm for the three caudal sites along the dorso-ventral axis of the hippocampus in this coronal plane. A total of 5 × 104 MGE cells in a 0.5 µl volume were delivered to each target coordinate (0.5 µl/1 min). Animals were removed from the stereotaxic instrument, and an intraperitoneal injection (ip) of buprenorphine (0.03 mg/kg) was administered as an analgesic medication. Topical triple antibiotic ointment was applied
