Vectors encoding myc-tagged FoxO1-WT, FoxO1-ADA (constitutively nuclear form with the mutations T24A, S253D and T316A) and FoxO1-DN (dominant-negative form lacking trans-activation domain with a deletion truncating after amino acid 265) and control vector, pCMV5, were used for FoxO1 overexpression experiments. FoxO1-specific siRNAs purchased from Bioneer (Bioneer Corp., Seoul, Korea, Cat.No. 1058762 and Cat.No. 1058758 for human FoxO1 siRNAs) and from Dharmacon (Dharmacon Inc., CO, USA, Cat.No. J-041127-08 and Cat.No. J-041127-07 for mouse FoxO1 siRNAs) were used to knockdown FoxO1.
