HEK293T and Neuro2A cells obtained from American Type Culture Collection (ATCC, VA, USA) were maintained in DMEM media containing 10% fetal bovine serum and 1% penicillin/streptomycin. Cells were transfected with Lipofectamine 2000 (Invitrogen, CA, USA) according to the manufacturer's instructions. For luciferase assay, cells were lysed 24 h after transfection. For mRNA and protein levels, cells were collected 48–96 h after transfection. All transfections were performed in triplicate.
