Paired-end sequencing (50/35 bps) fragments from ribosome-depleted RNA, spanning an average of ~35 million alignments per sample, were collected from the prefrontal cortex (PFC) of 16 alcoholics and 15 matched controls. Following filtering of raw reads for sequencing quality, RNA expression was detected using the RefSeq database 31 for 17,608 gene models, 29,607 alternatively spliced transcript models, and 196,453 exons. Alcohol dependent individuals varied in the duration and lifetime amount of alcohol consumed (Fig. S1); ranging from 20,857 to 325,893 standard drinks throughout the lifetime duration of alcohol dependent consumption. Alcohol dependent subjects did not significantly differ from control subjects in age, post-mortem interval (PMI), brain pH level, brain weight, RNA quality (RIN), or number of sequence alignments (Fig. S2).
