Chunk #19 — RESULTS — In parallel, dietary zinc supplementation in vivo improved both PU.1 and Nrf2 nuclear binding in alveolar macrophages from alcohol-fed rats
Therefore, we next isolated alveolar macrophages from control-fed rats as well as from alcohol-fed rats whose diets were supplemented with zinc acetate (100 mg/L) and performed electrophoretic mobility shift assays for PU.1 and Nrf2. As shown in Figure 3, both PU.1 (panel A) and Nrf2 (panel B) nuclear binding were decreased in alveolar macrophages from alcohol-fed rats compared to control-fed rats (Lane 2 vs. Lane 1 in each panel). However, consistent with the effects of zinc treatment in vitro in Figure 2, dietary zinc supplementation restored both PU.1 (panel A) and Nrf2 (panel B) nuclear binding in alveolar macrophages from alcohol-fed rats (Lane 3 vs. Lane 2 in each panel).
