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Chunk #9 — METHODS AND MATERIALS — Laboratory Procedures

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A Prospective 5-Year Re-examination of Alcohol Response in Heavy Drinkers Progressing in Alcohol Use Disorder.
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Starting at experimental time 0, the participant consumed his/her beverages presented in lidded, clear plastic cups in two equal portions with each portion consumed over 5 minutes separated by a 5 minute rest with the research assistant present(45, 46, 53). The average total beverage volume was 471 mL containing 190-proof ethanol (1% volume for placebo as a taste mask, 16% volume for alcohol beverage). The beverage was prepared with water, a flavored drinking mix, and a sucralose-based sugar substitute. Doses for women were 85% of those of men to adjust for sex differences in total body water(54, 55). Dependent measures and breathalyzer tests (Alco-Sensor IV, Intoximeter; St. Louis, MO) were repeated at 30, 60, 120, and 180 minutes. Other objective responses were obtained after the subjective measures and these results are presented elsewhere(53, 56). Breathalyzer readings were programmed to read 0.000 mg/dl, with actual values downloaded later. At the end of each session, after breath alcohol concentration (BrAC) was <40 mg/dl(57), the participant was transported to his/her lodging using a car service.