In public domain databases, many SNPs are mapped to different positions in the human genome. Therefore in the function report, we provide buttons for users to invoke agents to check the sequence quality of SNPs and verify that a SNP uniquely maps to one position in the human genome. One of these agents maps the SNP on the UCSC Golden Path (25) sequence and integrates both NCBI and Ensembl annotations. FASTSNP also uses NCBI BLAST (26) to search the UCSC Golden Path to extend the SNP sequence to 500 base-pairs. We do this because a SNP's sequence in public domain databases is usually too short to allow the design of good quality primers.
