Single-molecule fluorescent in situ hybridization experiments confirmed that, as predicted, expression of Pvalb, Syt2, and Nefm were highly correlated among Pvalb+ neurons in the frontal cortex (Figure 3E–G). Furthermore, Neurofilament IC cell loading was visible among Pvalb+ interneurons following Drop-seq analysis of 28,194 single nuclei isolated from flash-frozen frontal cortex (Figure 3H). These experiments confirm that the Neurofilament signal is present in vivo and not an artifact of cell isolation.
