Patch electrodes (3–6 ΩM) were pulled on a Flaming/ Brown microelectrode puller (Sutter Instruments) and filled with internal solution containing in mM: K-gluconate 70, KCl 80, EGTA 1, HEPES 10, Na-ATP 4, Na-GTP 0.3, QX-314 2, with osmolarity adjusted to 285–290 mOsm. Visually identified pyramidal neurons within the BLA were used for electrophysiological studies. Recordings were made using an Axopatch 1D amplifier (Axon instruments). Recordings were made at a holding potential of −70 mV. Evoked IPSCs (eIPSCs) were elicited by a bipolar stimulating electrode placed in the BLA with stimulation intensities varied from 3 to 40 V. eIPSP amplitudes were typically adjusted to 150–500 pA. Data were recorded using PClamp 9.2 (Molecular Devices).
