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Chunk #17 — METHODS — Electrophysiology

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Repeated homotypic stress elevates 2-arachidonoylglycerol levels and enhances short-term endocannabinoid signaling at inhibitory synapses in basolateral amygdala.
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For CB1 agonist and antagonist experiments, evoked simulations were elicited at 0.1 Hz with six stimulations being averaged to obtain one data point per minute. For depolarization-induced suppression of inhibition (DSI) studies, stimulations were made at 0.33 Hz. Access resistance (Ra) was monitored online using a 5 mV voltage step preceding each stimulation. Cells that showed increases in Ra of > 20% during an experiment or DSI trial or had an Ra >35 mΩ were excluded from the analysis.