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Chunk #18 — METHODS — Statistical Analysis

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Repeated homotypic stress elevates 2-arachidonoylglycerol levels and enhances short-term endocannabinoid signaling at inhibitory synapses in basolateral amygdala.
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Statistical analyses were performed using Prism Graphpad (San Diego, CA). Differences in 2-AG, AA, OAG, and SAG were evaluated by ANOVA followed by Dunnett’s test post hoc. For drug and stress DSI time-course experiments, data were analyzed by repeated measures ANOVA factoring time and treatment (drug or stress). For DSI summary data, we divided the postdepolarization period into a maximal DSI (average amplitude of the first three eIPSCs after the depolarizing pulse) and a late DSI (average amplitude of the last 10 eIPSCs of the experiment) component, which were compared with the average amplitude of the 10 baseline eIPSCs. Selected comparisons of summary DSI data were analyzed by ANOVA followed by Bonferroni’s test post hoc. Typically, data from two to three DSI trials per cell were averaged before analysis. p < 0.05 was considered significant throughout. Data are presented as mean ± SEM.