Taqman-based real-time PCR was used to confirm exon-level expression changes. Primers and fluorescently-labeled probes were custom designed for specific detection of exon-level expression. The follow primers/probes were used: ULK4, TCTCGTCCTAAAGCTTCTTCAGATT; ULK4, CTTTTCTGAGGATCTCTTTGAAGT; ULK4.PROBE, VIC- ATTAATTTGCTTGATGGGTT; SLC12A7F, ATCCTGGGCGTCATCCTCT; SLC12A7R, CACATGGCCACGATGAGG; SLC12A7.PROBE, VIC-CTGGTGTCCTGGAGTCCT; KLHL24F, TGGTACTAATATTGGGACGCAGAC; KLHL24R, CGCTTAGTTGCTGGGGAATC; KLHL24.PROBE, VIC- TAAACAGAGAGGATCTTGGG.
