Using this approach, increased methylation in 1,028 genes was observed after alcohol treatment (without consideration of neural tube phenotype), and 1,136 genes showed decreased methylation. When considering the different phenotypes, 1,126 genes displayed increased methylation in the ALC-NTC group, and decreased methylation of 1,030 genes was observed. In the ALC-NTO group, increased methylation of 1,045 genes was seen, and 1,202 genes displayed decreased methylation (p < 0.01). As shown in the Venn diagram in Figure 4, an intersection of the two analyses revealed increased methylation common of 147 genes (Suppl. S1) and decreased methylation of 141 genes (Suppl. S2).
