Since we showed that activity of mushroom body neurons was required for the memory of ethanol reward, we were interested in identifying genes that may act in the mushroom body to regulate these memories. To investigate this, we tested memory for ethanol reward 24 hrs after training in 160 strains with GAL4 reporter expression in the mushroom body. We found 3 mutations that resulted in persistent conditioned aversion, 54 mutations that resulted in lack of conditioned preference, and 3 mutations that resulted in enhanced conditioned preference (Fig. 7a).
