IPA identified 114 pathways that met FDR < 0.20 and contained at least 5 differentially expressed genes (Supplementary Table S2). [Several of the pathways were redundant, sharing all or most of their genes with another pathway.] The most significant pathway that was affected by CIE was cholesterol biosynthesis; the rate-limiting gene for cholesterol production, HMGCR, and 10 other genes in that pathway showed decreased expression after CIE. Pathways that showed overall activation (i.e., had positive z-scores) include dopamine receptor signaling, TGF-β signaling, and synaptogenesis signaling. Other affected pathways important in neurons were reelin signaling in neurons, axonal guidance, Ephrin A signaling, and GABA receptor signaling.
