Next, we addressed if alcohol affects the expression of splicing factors. First, using Gene Set Enrichment Analysis resource, we have compiled the list of all spliceosomal factors. In total, 127 factors were included (Supplementary Table). Then, we undertook an animal study in which we treated rats with alcohol for 7 weeks (5 days a week, 14 h a day). At the end of the experiment, animals were sacrificed, RNA was isolated from the whole brain, and transcriptome was profiled using RNA sequencing. Among all splicing factors, only SF1 (splicing factor 1) was altered, demonstrating ~50% decrease in rats exposed to alcohol (data not shown). Of note, only four genes were differentially expressed in response to alcohol which differs much from relatively broader transcriptomic changes detected when brain regions were studied individually (Fig. 1). Such a discrepancy likely supports the concept that examination of the RNA profiles from the whole brain may be inaccurate due to region-specific transcriptomic alterations and that the RNA profiling in the brain should rather be done in a region-specific or/and lineage-specific manner. Next, we interrogated RNA
