is not elucidated. We initially studied the expression of canonical snRNAs transcripts: snU1, snU4, snU6, and snU7 (expression of other canonical snRNA transcripts such as snU2, snU4atac, snU6atac, snU11, and snU12 was not reliably detected in our RNA sequencing output). We found that these 4 snRNAs were not affected in all studied brain regions (P > 0.05, Fig. 3). As each snRNAs has slightly divergent copies in the genome (sometimes referred to as snRNA pseudogenes), and some of them appear to be functional25,26, we additionally studied the expression of whole snRNAomes. As with canonical snRNAs, we found no difference in snRNAomes in all four regions between control subjects and patients with AUD (P > 0.05, Supplementary Fig. 3).
