Two studies using Illumina’s 27k platform were found. The first, using a small sample size of 20 alcohol dependent cases versus controls [97] reported differential methylation occurring within individuals between two time points at 252 genes in controls, 200 in cases, and 3 in both. Unfortunately, the published article does not provide the specific gene list or corresponding P values (noting only that the reported genes had p values < 0.01). The second study [98] had a larger sample size of 128 participants, and reported significant associations at 1710 CpG sites (p < 0.005 after Benjamini-Hochberg correction and delta beta ≥ 17%), also reporting that all of the 50 sites were hypomethylated. The authors report the differentially methylated loci to include two alcohol dehydrogenases, one aldehyde dehydrogenase, and CYP2A13, and five loci with delta beta values over 40%, including C8orf4, HCRTR1, FLJ38379, HSA277841, and TSC2, but do not include a list of methylation values, p values for association in the publication or supplemental materials.
