Samples were imaged using a Nikon C1si Spectral Confocal (Nikon Imaging Center @ Q3B, UCSF, San Francisco, CA). Images were acquired using a 20X objective and scanned at a resolution of 1024 × 1024 pixels. Images were prepared using Nikon EZ-C1, NIS C for C1 analysis software (http://www.nikon-instruments.jp). Adobe Photoshop CS was used to tile images and to enhance contrast on whole images. Mushroom body expression was analyzed similarly to previous MB-GAL4 characterizations45.
