RNA was extracted from 4 day-old adult flies by homogenization in Trizol (Invitrogen) and stored in -80°C. Samples were treated with DNase (Promega RQ1 RNase-free DNAse M6101) and cDNA was synthesized using Applied Biosystems TaqMan reverse transcription reagents (ABS N808-0234). Quantitative RT-PCR was performed using TaqMan Universal PCR Master Mix (no AmpErase UNG, ABS432018) on an Applied Biosystems 7900HT Fast Real-Time PCR System. Primers and probes recognizing sca (Dm01793316_g1) and Rpl32 (Dm02151827_g1) were designed by and obtained from Applied Biosystems.
