All behavioral data during alcohol SA, including FR, PR, extinction, and relapse, were analyzed using repeated measures analysis of variance (ANOVA). When P values reached level of significance (P < 0.05), further analysis was performed using one‐way ANOVA, paired or unpaired student's t test, and post hoc Tukey' honestly significant difference (HSD) multiple comparisons. Homogeneity of variance was estimated, and Hyunh‐Feldt correction or nonparametric Kruskal‐Wallis H test was implemented in case of assumption violation. All statistics were performed using IBM SPSS Statistics 24. In the alcohol SA paradigm, one animal (control) was excluded from statistical analysis as behavioral outlier (>2 × SD from mean) in greater than 50% of the FR3 and greater than 50% of the PR sessions.
