AZA also affected the intracellular distribution of epigenetic marks. AZA treatment changed the intracellular distribution of migrated cells in 5-MeC. 5-MeC, originally only perinuclear, was found throughout the nucleus, but excluded from the nucleoli. Some cells with perinuclear distribution of 5-MeC appeared to be at the initial stage of mitosis. Although the expression profile of DNMT1 was altered due to the AZA treatment, the intracellular distribution of DNMT1 did not change.
