The AZA treatment resulted in a lack of, or decline in, migration and differentiation of NSCs. The number and distance of NSCs that migrated out of neurospheres were evidently decreased (Figure 5E vs 5F) and the NSCs that did migrate were not well differentiated (as indicated by their morphology). Quantifying the number of migrated cells in the migrated zone of control and AZA treated cultures revealed that AZA significantly (p< 0.05) reduced migration, accounting for more than a 70% decrease in the number of cells migrating from neurospheres treated with AZA compared to controls (Figure 5E, F, Table 3).
