The 5-aza-cytidine (AZA) treatment altered histone and DNA methylation, although preferentially affected DNA methylation. AZA significantly increased (p-value < 0.05) the intensity of 3me-H3K27 in the migrated cells, compared to the differentiating migrated control (Figure 5A and B), but did not affect 2me-H3K4 (Ac-H4 was not studied). AZA treatment disrupted the mosaic pattern of 5-MeC in the core and considerably altered the normal ring-shaped distribution of DMNT1 found in the periphery of neurospheres (Figure 5C, D). DMNT3a-im cells notably declined in all zones of differentiation, compared to their respective differentiated controls (Table 1).
