Ac-H4 avoided the center of the nucleus (presumably nucleolus) in undifferentiated NSCs, but filled it in all cells upon differentiation. 2me-H3K4 was found in the nucleus, similarly circumventing supposed nucleolus (Figure 4E, F), while 3me-H3K27 exhibited a characteristic granule-like staining throughout the nucleus (Figure 2K, L arrowheads). Nuclear localization of 2me-H3K4 and 3me-H3K27 was not changed upon differentiation or migration. However, a clear transfer of the histone mark from parental cell to daughter cells was demonstrated during mitosis; the 2me-H3K4 marks divided into two comparable amounts, associating with DNA (indicated by DAPI) in two progeny (Figure 2G).
