The PPARα agonist fenofibrate (feno) (Sigma-Aldrich, St. Louis, MO), pan-PPAR agonist bezafibrate (beza) (Sigma-Aldrich, St. Louis, MO) and dual PPARα-PPARγ agonist tesaglitazar (tesa) (Tocris Bioscience, Minneapolis, MN) were freshly prepared as suspensions in saline with 4–5 drops of Tween-80 and injected in a volume of 0.05 ml/10 g of body weight. We administered (p.o.) PPAR agonist (150 mg/kg, 75 mg/kg, or 1.5 mg/kg of feno, beza, and tesa, respectively) or saline for eight days. Doses of drugs and routes of administration were based on published biological activity in vivo. Tesa was only delivered for six days in the 2BC experiment because its decreased effect on ethanol drinking behavior plateaued at that time-point.
