We used a two-bottle choice (2BC) voluntary ethanol consumption protocol as previously described 8. Briefly, two drinking bottles were continuously available to individually-housed mice. One contained water and the other 15% ethanol (v/v). Once stable ethanol consumptions were reached, we measured ethanol intake after two days of saline injections and grouped mice (8 mice per group) to provide similar levels of ethanol intake and preference. We administered PPAR agonists or saline depending on their group assignment. We measured consumption (g/kg body weight/24 hours) and calculated preference as the amount of ethanol consumed divided by the total amount of fluids consumed per day (a value > 50% indicates a preference for ethanol). Data are reported as the mean ± S.E.M. We used the statistics software program GraphPad Prism (Jandel Scientific, Costa Madre, CA) to perform a two-way ANOVA with repeated measurements and Bonferroni post hoc test.
