Chunk #6 — Materials and methods — Tissue extraction

Source

Critical role of cytochrome P450 2E1 (CYP2E1) in the development of high fat-induced non-alcoholic steatohepatitis.

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Total homogenate from each mouse was prepared in extraction buffer (50 mM Tris-Cl, pH 7.5, 1 mM EDTA, and 1% CHAPS), which was pre-equilibrated with nitrogen gas to remove the dissolved oxygen and liver homogenates were prepared as previously described [15,16].