Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were monitored in each mouse using the clinical IDEXX Vet Test chemistry analyzer system (IDEXX Laboratories, West Brook, ME, USA). CYP2E1 activity levels were measured by the rate of p-nitrophenol (PNP) oxidation to p-nitrocatechol, as described [15]. The concentration of MDA+HAE (μM) was measured using commercially available kits (Oxford Biomedical Research, Oxford, MI, USA) following the manufacturer’s protocol. Intrahepatic TNFα and MCP-1 levels (in 0.5 mg liver extracts) were determined by ELISA using the manufacturer’s protocols (Thermoscientific, Rockford, IL, USA).
