Chunk #8 — Materials and methods — Protein oxidation and glycoprotein determination

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Critical role of cytochrome P450 2E1 (CYP2E1) in the development of high fat-induced non-alcoholic steatohepatitis.

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The levels of carbonylated proteins were determined with the OxyBlot protein oxidation detection kit following the manufacturer’s protocol (Millipore, Billerica, MA, USA). Glycoproteins were evaluated using Pro-Q Emerald 488 Dye following the manufacturer’s protocol (Molecular Probes, Eugene, OR, USA).