Analyses of the effects of restraint stress on the tissue contents of AEA and 2-AG, CB1 receptor binding parameters and CB1 receptor mediated GTPγS binding were performed using independent t-tests. Examination of the effects of URB597 on stress-induced corticosterone secretion was performed using a one way analysis of variance (ANOVA), while the effects of HU-210 and AM251 on basal and stress-induced corticosterone secretion were analyzed using a univariate ANOVA with drug (HU-210 or AM251) and stress as fixed factors. For all neuroendocrine studies, post-hoc analysis was performed using a Tukey's test. All analyses used p < 0.05 as an indication of significance.
