NEAT1 transcript after 1 and 3 hours (Supplementary Fig. 2b). We validated that the SH-SY5Y cells were activated following KCl administration via the expected increase in transcript levels of immediate early genes (IEGs) FOS and ARC (Supplementary Fig. 3). Furthermore, we show by immunohistochemistry that KCNAB2 protein is mostly nuclear in inactivated cells (Fig. 1e) but becomes mostly cytoplasmic after 3 hours post KCl activation (Fig. 1f) supporting our western blot analysis data.
