To investigate the link between NEAT1, potassium channel proteins, and seizure risk in a relevant human cellular system, we generated functional human cortical-type neurons from human induced pluripotent stem cells (iPSCs) [Supplementary Fig. 4a–e6]. Robust cortical differentiation21 was verified using a panel of neuronal markers, showing that the in vitro neuronal differentiation protocol recapitulated major in vivo cortical differentiation stages (Supplementary Fig. 5a–c). MAP2 immunoreactivty revealed extensive neural fiber networks present in mature cultures (Supplementary Fig. 5d,e). We found that NEAT1 expression is up-regulated during iPSC-derived neuronal differentiation (Fig. 2a) and is localized to the nucleus in mature iPSC-derived neurons, as expected8, using fluorescence in situ hybridization (Supplementary Fig. 5f).
