Chunk #130 — STAR★Methods — Quantification and Statistical Analysis — Trinarization

Source

Molecular Architecture of the Mouse Nervous System.

Embedded

yes

Text

It is often useful to estimate (for each cluster) if a gene is likely expressed, not expressed, or we are not sure. That is, we want to trinarize the raw expression data into calls of expressed, not expressed, and indeterminate. Here we used a Bayesian beta-binomial model to trinarize the raw data.