TELiS analysis utilizing default optimal parameter settings (Table 3) also identified several other signaling alterations associated with social isolation, including: activation of STAT family transcription factors that mediate signaling through multiple cytokine and growth factor receptors; reduced activity of IRF1, which responds specifically to type I interferons (consistent with the previously noted down-regulation of interferon response genes); and reduced activity of GATA family transcription factors. These results emerged as statistically significant in primary analyes, but were sporadically non-significant when TELiS analytic parameters were parametrically varied [41]. Indications of STAT, IRF1, and GATA alterations should, therefore, be considered provisional. Among 192 vertebrate transcription control motifs analyzed by TELiS, no others showed consistent differential activity in association with subjective social isolation.
