To assess the reproducibility of the differentiation protocol, we induced microglia from 20 different ES and iPS cell lines representing a panel of healthy and disease genotypes (Supplementary Table 2), and successfully generated pMGLs from all lines. Supplementary table 2 reports quantitative output at an early cutoff of 4 weeks, matching the cutoff for NPCs. The cumulative yields are significantly higher. For example, after 8 weeks of differentiation, hES-wibr1, iPS-wt1, iPS-wt4, iPS-ALD4 and iPS-ALD1 respectively generated 1×106, 1×106, 3×106, 2×106, and 8×106 microglia-like cells, from 2×106 pluripotent stem cells at the onset. We conclude that the differentiation protocol is efficient and robust, though the genetic background of the pluripotent donor affects the kinetics and yield of the microglia-like cells.
