Initially, EdU incorporation was seen in 60% of nuclei from early pMGLs (2 weeks from onset of production). After 2 months in culture, EdU incorporation showed that less than 5% of pMGLs kept in MGdM were mitotic within a 24-hour period (Fig. 2h). Importantly, both primary fetal microglia and pMGLs could be maintained for several months in culture despite ultimately limited renewal potential, demonstrating their longevity.
